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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Melanocortin-2 receptor accessory protein,MRAP
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Melanocortin- 2 receptor accessory protein, MRAP ELISA KIT
Product Name:Human Melanocortin- 2 receptor accessory protein, MRAP ELISA KIT
Packing:96T
Catalog No.:ELI-42885h
Gene Name:MRAP
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Melanocortin-2 receptor accessory protein(MRAP
Alternative Name:MRAP,Melanocortin-2 receptor accessory protein(MRAP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Melanocortin- 2 receptor accessory protein, MRAP ELISA KIT allows for the in vitro quantitative determination of Melanocortin-2 receptor accessory protein(MRAP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Melanocortin- 2 receptor accessory protein, MRAP ELISA KIT has been pre-coated with an Melanocortin-2 receptor accessory protein(MRAP antibody specific to Melanocortin-2 receptor accessory protein(MRAP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Melanocortin-2 receptor accessory protein(MRAP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Melanocortin-2 receptor accessory protein(MRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Melanocortin-2 receptor accessory protein(MRAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Characterization of Melanocortin Receptors
I]NDP‐α‐MSH equilibrium binding for cloned human melanocortin receptor subtypes with time. Data are expressed as specific binding ( B o – NSB) versus time in hours. Each curve was generated by repetitive plate counting at specific time points. Data
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Overview of Receptor Allosterism
, which recognizes endogenous ligands, most G protein?coupled receptors (GPCRs) possess topographically distinct allosteric sites that can be recognized by small molecules and accessory cellular proteins. Ligand binding to allosteric sites promotes a conformational
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