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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
B- cell linker protein, BLNK
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human B- cell linker protein, BLNK ELISA KIT
Product Name:Human B- cell linker protein, BLNK ELISA KIT
Packing:96T
Catalog No.:ELI-05235h
Gene Name:BLNK
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:B-cell linker protein(BLNK
Alternative Name:BLNK,B-cell linker protein(BLNK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human B- cell linker protein, BLNK ELISA KIT allows for the in vitro quantitative determination of B-cell linker protein(BLNK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human B- cell linker protein, BLNK ELISA KIT has been pre-coated with an B-cell linker protein(BLNK antibody specific to B-cell linker protein(BLNK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for B-cell linker protein(BLNK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain B-cell linker protein(BLNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of B-cell linker protein(BLNK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
CellTrace CFSE Cell Proliferation Kit
实验材料 Kit Contents CellTrace™ CFSE (Component A), 10 vials, each containing 50 μg of lyophilized powder DMSO (Component B), 1 vial containing 0.5 mL of high quality dimethylsulfoxide
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