- ￥2890
- LSM Bio
- 0.312-20ng/ml
- 0.1ng/ml
- 进口
- ELI-41119h
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.312-20ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Rho GTPase-activating protein 20,ARHGAP20
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human Rho GTPase- activating protein 20, ARHGAP20 ELISA KIT
Product Name:Human Rho GTPase- activating protein 20, ARHGAP20 ELISA KIT
Packing:96T
Catalog No.:ELI-41119h
Gene Name:ARHGAP20
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Rho GTPase-activating protein 20(ARHGAP20
Alternative Name:ARHGAP20,Rho GTPase-activating protein 20(ARHGAP20
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase- activating protein 20, ARHGAP20 ELISA KIT allows for the in vitro quantitative determination of Rho GTPase-activating protein 20(ARHGAP20 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase- activating protein 20, ARHGAP20 ELISA KIT has been pre-coated with an Rho GTPase-activating protein 20(ARHGAP20 antibody specific to Rho GTPase-activating protein 20(ARHGAP20 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase-activating protein 20(ARHGAP20 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase-activating protein 20(ARHGAP20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase-activating protein 20(ARHGAP20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验和抗体配对最早于20世纪80年代被发现，当时科学家将Rho1D4抗体交联包被在琼脂糖凝胶上，用来纯化通过猴肾细胞表达的牛视紫红质。1,2从那时起，Rho1D4系统开始被广泛的用于小量膜蛋白提取的研究中，包括GPCR（G蛋白偶联受体），ATP结合盒转运蛋白（ATP-binding cassette transporter），溶质反向转运体和以及一种含四个跨膜域的膜蛋白。3） Rho1D4系统的优势和用途高纯度Rho1D4系统包含：标签、抗体偶联亲和基质（琼脂糖或磁珠等）以及洗脱多肽。Rho
Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
., and Danuser, G. 2009. Coordination of Rho GTPase activities during cell protrusion. Nature 461:99‐103. Michaelson, D., Silletti, J., Murphy, G., D'Eustachio, P., Rush, M
in 1971, it contained only seven structures (Berman, et al., 2000). Since then, the pace at which protein structures are being discovered has grown exponentially, with the PDB currently containing over 20,000 structures (PDB, 2003). Our ability
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