Human SLC50A1 ELISA Kit
Product Name:Human SLC50A1 ELISA Kit
Packing:96T
Catalog No.:ELA-E15030h
Gene Name:SLC50A1
Detect Range:0.156-10 ng/mL
Sensitivity:0.098ng/ml
Target Protein Name:Human Sugar transporter SWEET1
Alternative Name:Sugar transporter SWEET1, HsSWEET1, RAG1-activating protein 1, Solute carrier family 50 member 1, Stromal cell protein, SLC50A1, RAG1AP1, SCP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human SLC50A1 ELISA Kit allows for the in vitro quantitative determination of Human Sugar transporter SWEET1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human SLC50A1 ELISA Kit has been pre-coated with an SLC50A1 antibody specific to Human Sugar transporter SWEET1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Sugar transporter SWEET1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Sugar transporter SWEET1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Sugar transporter SWEET1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!