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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human NKG2-F type II integral membrane protein,KLRC4
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human NKG2- F type II integral membrane protein, KLRC4 ELISA KIT
Product Name:Human NKG2- F type II integral membrane protein, KLRC4 ELISA KIT
Packing:96T
Catalog No.:ELI-39616h
Gene Name:KLRC4
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:NKG2-F type II integral membrane protein(KLRC4
Alternative Name:KLRC4,NKG2-F type II integral membrane protein(KLRC4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NKG2- F type II integral membrane protein, KLRC4 ELISA KIT allows for the in vitro quantitative determination of NKG2-F type II integral membrane protein(KLRC4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NKG2- F type II integral membrane protein, KLRC4 ELISA KIT has been pre-coated with an NKG2-F type II integral membrane protein(KLRC4 antibody specific to NKG2-F type II integral membrane protein(KLRC4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NKG2-F type II integral membrane protein(KLRC4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NKG2-F type II integral membrane protein(KLRC4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of NKG2-F type II integral membrane protein(KLRC4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
cleavable signal sequence and for type II integral membrane proteins with a cytosolic C‐terminus. (C ) pBT3‐N bait vector for type II integral membrane proteins with a cytosolic N‐terminus. (D ) pDHB1 membrane anchor bait vector for soluble bait proteins
Laurdan Studies of Membrane Lipid-Nicotinic Acetylcholine Receptor Protein Interactions
, namely its ability to act as a F�rster-type resonance energy transfer (FRET) acceptor of tryptophan emission, was used to learn about the physical state of lipids within F�rster distance from donor tryptophan residues in integral membrane proteins. The application
acquisition to optimize imaging of fluorescence resonance energy transfer by sensitized emission. Biophys. J. 86:2517‐2529. Wouters, F.S. and Bastiaens, P.I.H. 2001. Imaging protein
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