- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-38929h
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Rho GTPase-activating protein 26,ARHGAP26
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Rho GTPase- activating protein 26, ARHGAP26 ELISA KIT
Product Name:Human Rho GTPase- activating protein 26, ARHGAP26 ELISA KIT
Packing:96T
Catalog No.:ELI-38929h
Gene Name:ARHGAP26
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Rho GTPase-activating protein 26(ARHGAP26
Alternative Name:ARHGAP26,Rho GTPase-activating protein 26(ARHGAP26
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase- activating protein 26, ARHGAP26 ELISA KIT allows for the in vitro quantitative determination of Rho GTPase-activating protein 26(ARHGAP26 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase- activating protein 26, ARHGAP26 ELISA KIT has been pre-coated with an Rho GTPase-activating protein 26(ARHGAP26 antibody specific to Rho GTPase-activating protein 26(ARHGAP26 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase-activating protein 26(ARHGAP26 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase-activating protein 26(ARHGAP26, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase-activating protein 26(ARHGAP26 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验蛋白可诱导的HEK293S细胞系（哺乳动物）＞95%26μg/3×107, 个细胞Rho1D4 IACAE1 溶质载体S.啤酒系BJ545793%2.5mg/18L. 培养物Rho1D4 IAC*纯度是从低浓度经一步Rho1D4 IAC在反应溶液中得出的；产率是在洗脱成分浓缩并过SEC柱后计算的表1：有文献报道的使用Rho1D4系统纯化膜蛋白的纯度和产率。尽管很多用Rho1D4系统纯化的膜蛋白均为G蛋白偶联受体家族，该系统也可以有助于辨别其他如转运体之类的膜蛋白。相关文章在参考文献中。IAC
Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
and image analysis. Curr. Protoc. Cell Biol. 46:14.11.1?14.11.26. © 2010 by John Wiley & Sons, Inc. Keywords: biosensors; Rho family GTPases; FRET; live cell imaging
Overview of Protein Microarrays
. Hu, S., Xie, Z., Onishi, A., Yu, X., Jiang, L., Lin, J., Rho, H.‐s., Woodard, C., Wang, H., and Jeong, J.‐S. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor
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