- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-51327h
- 2025年12月23日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ubiquitin-conjugating enzyme E2 E3,UBE2E3
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human Ubiquitin- conjugating enzyme E2 E3, UBE2E3 ELISA KIT
Product Name:Human Ubiquitin- conjugating enzyme E2 E3, UBE2E3 ELISA KIT
Packing:96T
Catalog No.:ELI-51327h
Gene Name:UBE2E3
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Ubiquitin-conjugating enzyme E2 E3(UBE2E3
Alternative Name:UBE2E3,Ubiquitin-conjugating enzyme E2 E3(UBE2E3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin- conjugating enzyme E2 E3, UBE2E3 ELISA KIT allows for the in vitro quantitative determination of Ubiquitin-conjugating enzyme E2 E3(UBE2E3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin- conjugating enzyme E2 E3, UBE2E3 ELISA KIT has been pre-coated with an Ubiquitin-conjugating enzyme E2 E3(UBE2E3 antibody specific to Ubiquitin-conjugating enzyme E2 E3(UBE2E3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin-conjugating enzyme E2 E3(UBE2E3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-conjugating enzyme E2 E3(UBE2E3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin-conjugating enzyme E2 E3(UBE2E3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation
SUMO conjugation to protein substrates requires the concerted action of a dedicated E2 ubiquitin conjugation enzyme (Ubc9) and associated E3 ligases. Although Ubc9 can directly recognize and modify substrate lysine residues that occur
Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
biochemical and neuropathological observations that had suggested involvement of the ubiquitin-proteasome system (UPS) in PD. Based on cellular expression studies and biochemical in vitro experiments, several researchers ascribed an E3-type, E2-dependent
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