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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Calcineurin B homologous protein 2,CHP2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human Calcineurin B homologous protein 2, CHP2 ELISA KIT
Product Name:Human Calcineurin B homologous protein 2, CHP2 ELISA KIT
Packing:96T
Catalog No.:ELI-33704h
Gene Name:CHP2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Calcineurin B homologous protein 2(CHP2
Alternative Name:CHP2,Calcineurin B homologous protein 2(CHP2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Calcineurin B homologous protein 2, CHP2 ELISA KIT allows for the in vitro quantitative determination of Calcineurin B homologous protein 2(CHP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Calcineurin B homologous protein 2, CHP2 ELISA KIT has been pre-coated with an Calcineurin B homologous protein 2(CHP2 antibody specific to Calcineurin B homologous protein 2(CHP2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Calcineurin B homologous protein 2(CHP2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Calcineurin B homologous protein 2(CHP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Calcineurin B homologous protein 2(CHP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
Polyclonal Antibody Production 简单快捷制备多抗方法,可用于western、ELISA和免疫/b
Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. -------------------------------------------------------------------------------- Rabbit
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
Hoppe‐Seyler, F., Crnkovic‐Mertens, I., Denk, C., Fitscher, B.A., Klevenz, B., Tomai, E., and Butz, K. 2001. Peptide aptamers: new tools to study protein interactions. J. Steroid. Biochem. Mol. Biol. 78:105‐111
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