Human Butyrylcholinesterase,BC

Human Butyrylcholinesterase, BCHE ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

HGNC,BChE, BuChE,BCHE,pseudocholinesterase,plasma cholinesterase,CHE1,CHE2; acylcholine acylhydrolase; butyrylcholine esterase; choline esterase II; cholinesterase (serum) 2; cholinesterase 1; pseudocholinesterase, Butyrylcholinesterase

Search name

Human HGNC ELISA KIT ,Human BChE ELISA KIT ,Human BuChE ELISA KIT ,Human BCHE ELISA KIT ,Human Cholinesterase ELISA KIT, Human pseudocholinesterase ELISA KIT ,Human plasma cholinesterase ELISA KIT ,Human CHE1 ELISA KIT ,Human CHE2 ELISA KIT ,Human acylcholine acylhydrolase ELISA KIT ,Human butyrylcholine esterase ELISA KIT ,Human choline esterase II ELISA KIT ,Human cholinesterase (serum) 2 ELISA KIT ,Human cholinesterase 1 ELISA KIT ,Human pseudocholinesterase ELISA KIT ,Human Butyrylcholinesterase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human Cholinesterase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinesterase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cholinesterase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cholinesterase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cholinesterase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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