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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human F-box/LRR-repeat protein 15,FBXL15
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human F- box/LRR- repeat protein 15, FBXL15 ELISA KIT
Product Name:Human F- box/LRR- repeat protein 15, FBXL15 ELISA KIT
Packing:96T
Catalog No.:ELI-32542h
Gene Name:FBXL15
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:F-box(LRR-repeat protein 15(FBXL15
Alternative Name:FBXL15,F-box(LRR-repeat protein 15(FBXL15
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human F- box/LRR- repeat protein 15, FBXL15 ELISA KIT allows for the in vitro quantitative determination of F-box(LRR-repeat protein 15(FBXL15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human F- box/LRR- repeat protein 15, FBXL15 ELISA KIT has been pre-coated with an F-box(LRR-repeat protein 15(FBXL15 antibody specific to F-box(LRR-repeat protein 15(FBXL15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for F-box(LRR-repeat protein 15(FBXL15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain F-box(LRR-repeat protein 15(FBXL15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of F-box(LRR-repeat protein 15(FBXL15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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