- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-31220h
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human G kinase-anchoring protein 1,GKAP1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human G kinase- anchoring protein 1, GKAP1 ELISA KIT
Product Name:Human G kinase- anchoring protein 1, GKAP1 ELISA KIT
Packing:96T
Catalog No.:ELI-31220h
Gene Name:GKAP1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:G kinase-anchoring protein 1(GKAP1
Alternative Name:GKAP1,G kinase-anchoring protein 1(GKAP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human G kinase- anchoring protein 1, GKAP1 ELISA KIT allows for the in vitro quantitative determination of G kinase-anchoring protein 1(GKAP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human G kinase- anchoring protein 1, GKAP1 ELISA KIT has been pre-coated with an G kinase-anchoring protein 1(GKAP1 antibody specific to G kinase-anchoring protein 1(GKAP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for G kinase-anchoring protein 1(GKAP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G kinase-anchoring protein 1(GKAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of G kinase-anchoring protein 1(GKAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Meng, L., Michaud, G.A., Merkel, J.S., Zhou, F., Huang, J., Mattoon, D.R., and Schweitzer, B. 2008. Protein kinase substrate identification on functional protein arrays. BMC Biotechnol. 8:22.
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Manning, G., Whyte, D.B., Martinez, R., Hunter, T., and Sudarsanam, S. 2002b. The protein kinase complement of the human genome. Science 298:1912‐1934.
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