Product Name:Human G antigen 13, GAGE13 ELISA KIT Packing:96T
Catalog No.:ELI-30792h
Gene Name:GAGE13
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:G antigen 13(GAGE13
Alternative Name:GAGE13,G antigen 13(GAGE13 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human G antigen 13, GAGE13 ELISA KIT allows for the in vitro quantitative determination of G antigen 13(GAGE13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human G antigen 13, GAGE13 ELISA KIT has been pre-coated with an G antigen 13(GAGE13 antibody specific to G antigen 13(GAGE13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for G antigen 13(GAGE13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G antigen 13(GAGE13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of G antigen 13(GAGE13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!