Human Putative DNA repair and

Human Putative DNA repair and recombination protein RAD26- like, RAD26L ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ERCC6L2,RAD26L; C9orf102; SR278; excision repair cross-complementing rodent repair deficiency, complementation group 6-like 2; stretch responsive protein 278; excision repair cross-complementation group 6-like 2

Search name

Human ERCC6L2 ELISA KIT ,Human RAD26L ELISA KIT ,Human C9orf102 ELISA KIT ,Human SR278 ELISA KIT ,Human excision repair cross-complementing rodent repair deficiency ELISA KIT ,Human  complementation group 6-like 2 ELISA KIT ,Human stretch responsive protein 278 ELISA KIT ,Human excision repair cross-complementation group 6-like 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Putative DNA repair and recombination protein RAD26- like, RAD26L concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD26L, During the reaction, RAD26L in the sample or standard competes with a fixed amount of biotin-labeled RAD26L for sites on a pre-coated Monoclonal antibody specific to RAD26L. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RAD26L in the samples is then determined by comparing the O.D. of the samples to the standard curve.