- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-22153h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
DNA repair protein RAD50,RAD50
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human DNA repair protein RAD50, RAD50 ELISA KIT
Product Name:Human DNA repair protein RAD50, RAD50 ELISA KIT
Packing:96T
Catalog No.:ELI-22153h
Gene Name:RAD50
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:DNA repair protein RAD50(RAD50
Alternative Name:RAD50,DNA repair protein RAD50(RAD50
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human DNA repair protein RAD50, RAD50 ELISA KIT allows for the in vitro quantitative determination of DNA repair protein RAD50(RAD50 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human DNA repair protein RAD50, RAD50 ELISA KIT has been pre-coated with an DNA repair protein RAD50(RAD50 antibody specific to DNA repair protein RAD50(RAD50 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for DNA repair protein RAD50(RAD50 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DNA repair protein RAD50(RAD50, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of DNA repair protein RAD50(RAD50 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验In Vitro DNA Mismatch Repair in Human Cells
The in vitro DNA mismatch repair (MMR) assay is a very useful technique for studying the functions and the mechanisms of the MMR system in genome maintenance. This assay has been effectively used to evaluate MMR proficiency in various tumor
Strategies for Cloning Mammalian DNA Repair Genes
During the last 20 years, the cloning and identification of DNA repair genes in bacteria, yeast, human, mouse, and other organisms have been an expanding enterprise. Gene/cDNA isolation and analysis is a critical step toward discovering gene
Assay for Human Rad51-Mediated DNA Displacement Loop Formation
DNA (Stratagene) Plasmid Midi kit (QIAGEN) Proteinase K (Roche Applied Science) Recombinant Hop2-Mnd1 complex, purified (5 µM) or recombinant human Rad54, purified (5 µM) (see Step 5) Purify Hop2-Mnd1 complex
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