- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-30282h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Rho GTPase-activating protein 25,ARHGAP25
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Rho GTPase- activating protein 25, ARHGAP25 ELISA KIT
Product Name:Human Rho GTPase- activating protein 25, ARHGAP25 ELISA KIT
Packing:96T
Catalog No.:ELI-30282h
Gene Name:ARHGAP25
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Rho GTPase-activating protein 25(ARHGAP25
Alternative Name:ARHGAP25,Rho GTPase-activating protein 25(ARHGAP25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase- activating protein 25, ARHGAP25 ELISA KIT allows for the in vitro quantitative determination of Rho GTPase-activating protein 25(ARHGAP25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase- activating protein 25, ARHGAP25 ELISA KIT has been pre-coated with an Rho GTPase-activating protein 25(ARHGAP25 antibody specific to Rho GTPase-activating protein 25(ARHGAP25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase-activating protein 25(ARHGAP25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase-activating protein 25(ARHGAP25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase-activating protein 25(ARHGAP25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验-25 相关指标产品推荐： 重组蛋白&ELISA试剂盒 货号 产品名称 宿主/灵敏度 分子量/检测范围 P6652 Recombinant Human IL-25 E.Coli 18.6 kDa P6255 Recombinant Mouse IL-25 E.Coli 19.4 kDa EH0180 Human IL-17E/IL-25 ELISA Kit
Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
., and Danuser, G. 2009. Coordination of Rho GTPase activities during cell protrusion. Nature 461:99‐103. Michaelson, D., Silletti, J., Murphy, G., D'Eustachio, P., Rush, M
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
技术资料
