- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-30112h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human 11-cis retinol dehydrogenase,RDH5
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human 11- cis retinol dehydrogenase, RDH5 ELISA KIT
Product Name:Human 11- cis retinol dehydrogenase, RDH5 ELISA KIT
Packing:96T
Catalog No.:ELI-30112h
Gene Name:RDH5
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:11-cis retinol dehydrogenase(RDH5
Alternative Name:RDH5,11-cis retinol dehydrogenase(RDH5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human 11- cis retinol dehydrogenase, RDH5 ELISA KIT allows for the in vitro quantitative determination of 11-cis retinol dehydrogenase(RDH5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human 11- cis retinol dehydrogenase, RDH5 ELISA KIT has been pre-coated with an 11-cis retinol dehydrogenase(RDH5 antibody specific to 11-cis retinol dehydrogenase(RDH5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 11-cis retinol dehydrogenase(RDH5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 11-cis retinol dehydrogenase(RDH5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 11-cis retinol dehydrogenase(RDH5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Resuspend the bead-bound cells by thorough pipetting or vortex for 5 secs. 9) Add 1 ml Buffer 1. 10) Place the tube in the magnet for 3 min. 11) Transfer the supernatant to a new tube. 4. Downstream
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
The cellular retinaldehyde-binding protein (CRALBP) is expressed at high levels in vertebrate visual trssue, where it may serve to modulate the interaction of 11-cis -retinol with visual-cycle enzymes in the retinal pigment epithelium (RPE
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