- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-28636h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Ubiquitin carboxyl-terminal hydrolase 29,USP29
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Ubiquitin carboxyl- terminal hydrolase 29, USP29 ELISA KIT
Product Name:Human Ubiquitin carboxyl- terminal hydrolase 29, USP29 ELISA KIT
Packing:96T
Catalog No.:ELI-28636h
Gene Name:USP29
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Ubiquitin carboxyl-terminal hydrolase 29(USP29
Alternative Name:USP29,Ubiquitin carboxyl-terminal hydrolase 29(USP29
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin carboxyl- terminal hydrolase 29, USP29 ELISA KIT allows for the in vitro quantitative determination of Ubiquitin carboxyl-terminal hydrolase 29(USP29 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin carboxyl- terminal hydrolase 29, USP29 ELISA KIT has been pre-coated with an Ubiquitin carboxyl-terminal hydrolase 29(USP29 antibody specific to Ubiquitin carboxyl-terminal hydrolase 29(USP29 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin carboxyl-terminal hydrolase 29(USP29 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin carboxyl-terminal hydrolase 29(USP29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin carboxyl-terminal hydrolase 29(USP29 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
( protocol 34 ) E2‐UbcH5B ( protocol 37 ) GST‐ubiquitin ( protocol 29 ) Human APP‐BP1/Uba3 (NEDD8 E1; protocol 21 ) Ubc12 (E2
【分享】Human press METHODS IN MOLECULAR BIOLOGY
.Ubiquitin-Proteasome Protocols Edited by Patterson, Cam 2005 302.Handbook of ELISPOT Methods and Protocols Edited by Kalyuzhny, Alexander E. 2005 303.NanoBiotechnology Protocols Edited by Rosenthal, Sandra J 2005 304.Human Retrovirus Protocols Virology
A Method for Assaying Deubiquitinating Enzymes
). The purified Ub-specific protease-6 (yUBP-6) in yeast and cUBP41, Ub C-terminal hydrolase-1 (cUCH-1), cUCH-6, and cUCH-8 in chick skeletal muscle were prepared as described (13 , 15 -18 ). Ub-PESTc was purified from an E. coli strain AR13 carrying pNMHUB-PESTc
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