Human Ras GTPase- activating- like protein IQGAP1, IQGAP1 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
IQGAP1, IQGAP,ras GTPase-activating-like protein IQGAP1; HUMORFA01; SAR1; p195; RasGAP-like with IQ motifs; IQ motif containing GTPase activating protein 1
Search name
Human IQGAP1 ELISA KIT ,Human IQGAP ELISA KIT ,Human ras GTPase-activating-like protein IQGAP1 ELISA KIT ,Human HUMORFA01 ELISA KIT ,Human SAR1 ELISA KIT ,Human p195 ELISA KIT ,Human RasGAP-like with IQ motifs ELISA KIT ,Human IQ motif containing GTPase activating protein 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Ras GTPase- activating- like protein IQGAP1, IQGAP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IQGAP1, During the reaction, IQGAP1 in the sample or standard competes with a fixed amount of biotin-labeled IQGAP1 for sites on a pre-coated Monoclonal antibody specific to IQGAP1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IQGAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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