- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-27639h
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Lck-interacting transmembrane adapter 1,LIME1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Lck- interacting transmembrane adapter 1, LIME1 ELISA KIT
Product Name:Human Lck- interacting transmembrane adapter 1, LIME1 ELISA KIT
Packing:96T
Catalog No.:ELI-27639h
Gene Name:LIME1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Lck-interacting transmembrane adapter 1(LIME1
Alternative Name:LIME1,Lck-interacting transmembrane adapter 1(LIME1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Lck- interacting transmembrane adapter 1, LIME1 ELISA KIT allows for the in vitro quantitative determination of Lck-interacting transmembrane adapter 1(LIME1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Lck- interacting transmembrane adapter 1, LIME1 ELISA KIT has been pre-coated with an Lck-interacting transmembrane adapter 1(LIME1 antibody specific to Lck-interacting transmembrane adapter 1(LIME1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lck-interacting transmembrane adapter 1(LIME1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Lck-interacting transmembrane adapter 1(LIME1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Lck-interacting transmembrane adapter 1(LIME1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验AF006086 181-203 aagaatgaagctgataggacc Human HeLa View, Order View ATR interacting protein (4) AF451323 254-274 AAGGTCCACAGATTATTAGAT Human HeLa View, Order View ATR interacting protein (4)
VE-1 binding sites (comprising the 9 bp putative interacting sequence and three flanking base pairs) were determined by ELISA and normalized to VE-1 subs. The positions of the potential binding sites relative to the transcription start site
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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