Human Lymphoid- specific helicase, HELLS ELISA KIT
Product Name:Human Lymphoid- specific helicase, HELLS ELISA KIT Packing:96T
Catalog No.:ELI-27616h
Gene Name:HELLS
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Lymphoid-specific helicase(HELLS
Alternative Name:HELLS,Lymphoid-specific helicase(HELLS Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Lymphoid- specific helicase, HELLS ELISA KIT allows for the in vitro quantitative determination of Lymphoid-specific helicase(HELLS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Lymphoid- specific helicase, HELLS ELISA KIT has been pre-coated with an Lymphoid-specific helicase(HELLS antibody specific to Lymphoid-specific helicase(HELLS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Lymphoid-specific helicase(HELLS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Lymphoid-specific helicase(HELLS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Lymphoid-specific helicase(HELLS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!