Human Dual specificity protein phosphatase 13, DUSP13 ELISA KIT
Product Name:Human Dual specificity protein phosphatase 13, DUSP13 ELISA KIT
Packing:96T
Catalog No.:ELI-26134h
Gene Name:DUSP13
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Dual specificity protein phosphatase 13(DUSP13
Alternative Name:DUSP13,Dual specificity protein phosphatase 13(DUSP13
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Dual specificity protein phosphatase 13, DUSP13 ELISA KIT allows for the in vitro quantitative determination of Dual specificity protein phosphatase 13(DUSP13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Dual specificity protein phosphatase 13, DUSP13 ELISA KIT has been pre-coated with an Dual specificity protein phosphatase 13(DUSP13 antibody specific to Dual specificity protein phosphatase 13(DUSP13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Dual specificity protein phosphatase 13(DUSP13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dual specificity protein phosphatase 13(DUSP13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Dual specificity protein phosphatase 13(DUSP13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!