Human Adenylate cyclase type 1

Human Adenylate cyclase type 1, ADCY1 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Adenylate cyclase type 1,Ca(2+)/calmodulin-activated adenylyl cyclase,Adenylyl cyclase 1,ATP pyrophosphate-lyase 1,Adenylate cyclase
type I,

Search name
Human Adenylate cyclase type 1 ELISA Kit, Human ADCY1 ELISA Kit,Adenylate cyclase type 1 ELISA Kit, ADCY1 ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human adenylate cyclase type 1,Adenylate cyclase type 1
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate cyclase type 1. Standards or samples
are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Adenylate
cyclase type 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. Only those wells that contain Adenylate cyclase type 1, biotin-conjugated antibody and
enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Adenylate
cyclase type 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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