Human CASP8 and FADD-like apop

Human CASP8 and FADD- like apoptosis regulator, CFLAR ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CFLAR,FLIP,CASH; CASP8AP1; CLARP; Casper; FLAME; FLAME-1; FLAME1; I-FLICE; MRIT; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; FADD-like anti-apoptotic molecule; FADD-like antiapoptotic molecule 1; MACH-related inducer of toxicity; caspase homolog; caspase-eight-related protein; caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; cellular FLICE-like inhibitory protein; inhibitor of FLICE; usurpin beta; CASP8 and FADD-like apoptosis regulator, Usurpin, Caspase homolog,

Search name

Human CFLAR ELISA KIT ,Human FLIP ELISA KIT ,Human CASH ELISA KIT ,Human CASP8AP1 ELISA KIT ,Human CLARP ELISA KIT ,Human Casper ELISA KIT ,Human FLAME ELISA KIT ,Human FLAME-1 ELISA KIT ,Human FLAME1 ELISA KIT ,Human I-FLICE ELISA KIT ,Human MRIT ELISA KIT ,Human c-FLIP ELISA KIT ,Human c-FLIPL ELISA KIT ,Human c-FLIPR ELISA KIT ,Human c-FLIPS ELISA KIT ,Human FADD-like anti-apoptotic molecule ELISA KIT ,Human FADD-like antiapoptotic molecule 1 ELISA KIT ,Human MACH-related inducer of toxicity ELISA KIT ,Human caspase homolog ELISA KIT ,Human caspase-eight-related protein ELISA KIT ,Human caspase-like apoptosis regulatory protein ELISA KIT ,Human caspase-related inducer of apoptosis ELISA KIT ,Human cellular FLICE-like inhibitory protein ELISA KIT ,Human inhibitor of FLICE ELISA KIT ,Human usurpin beta ELISA KIT ,Human CASP8 and FADD-like apoptosis regulator ELISA KIT ,Human Usurpin ELISA KIT ,Human Caspase homolog ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human CASP8 and FADD-like apoptosis regulator, CFLAR concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CFLAR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CFLAR and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CFLAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CFLAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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