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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Protocadherin gamma-B2,PCDHGB2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Protocadherin gamma- B2, PCDHGB2 ELISA KIT
Product Name:Human Protocadherin gamma- B2, PCDHGB2 ELISA KIT
Packing:96T
Catalog No.:ELI-22838h
Gene Name:PCDHGB2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Protocadherin gamma-B2(PCDHGB2
Alternative Name:PCDHGB2,Protocadherin gamma-B2(PCDHGB2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protocadherin gamma- B2, PCDHGB2 ELISA KIT allows for the in vitro quantitative determination of Protocadherin gamma-B2(PCDHGB2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protocadherin gamma- B2, PCDHGB2 ELISA KIT has been pre-coated with an Protocadherin gamma-B2(PCDHGB2 antibody specific to Protocadherin gamma-B2(PCDHGB2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protocadherin gamma-B2(PCDHGB2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protocadherin gamma-B2(PCDHGB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protocadherin gamma-B2(PCDHGB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
. shilei5794749 呵呵,这个ELISA检测基因突变比较意思。刚刚看了几篇文章,跟楼主分享一下。 文章:Detection of four b-thalassemia point mutations in Iranians using a PCR-ELISA genotyping system. 检测图中四个位置的基因突变。这个检测方法的四要素:a,DIG labed PCR products b,5’biotinylated
ELISA KIT & ANTIBODY OF USCNLIFE
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