Human Serine/threonine-protein

Human Serine/threonine- protein kinase PINK1 mitochondrial, PINK1 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

PINK1,serine/threonine-protein kinase PINK1, mitochondrial; BRPK; PARK6; PTEN-induced putative kinase protein 1; protein kinase BRPK; PTEN induced putative kinase 1

Search name

Human PINK1 ELISA Kit ,Human serine/threonine-protein kinase PINK1 ELISA Kit ,Human mitochondrial ELISA KIT ,Human BRPK ELISA KIT ,Human PARK6 ELISA KIT ,Human PTEN-induced putative kinase protein 1 ELISA KIT ,Human protein kinase BRPK ELISA KIT ,Human PTEN induced putative kinase 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human Serine/threonine- protein kinase PINK1 mitochondrial, PINK1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PINK1, During the reaction, PINK1 in the sample or standard competes with a fixed amount of biotin-labeled PINK1 for sites on a pre-coated Monoclonal antibody specific to PINK1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PINK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.