- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-19564h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
NADP-dependent malic enzyme,ME1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human NADP- dependent malic enzyme, ME1 ELISA KIT
Product Name:Human NADP- dependent malic enzyme, ME1 ELISA KIT
Packing:96T
Catalog No.:ELI-19564h
Gene Name:ME1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:NADP-dependent malic enzyme(ME1
Alternative Name:ME1,NADP-dependent malic enzyme(ME1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NADP- dependent malic enzyme, ME1 ELISA KIT allows for the in vitro quantitative determination of NADP-dependent malic enzyme(ME1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NADP- dependent malic enzyme, ME1 ELISA KIT has been pre-coated with an NADP-dependent malic enzyme(ME1 antibody specific to NADP-dependent malic enzyme(ME1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NADP-dependent malic enzyme(ME1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NADP-dependent malic enzyme(ME1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of NADP-dependent malic enzyme(ME1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验为催化苹果酸脱羧反应的酶。正式名称称为苹果酸脱氢酶（脱羧作用）。已知有三种苹果酸酶（ EC1． 1． 1． 38— 40）。其中以 NADP为受体的酶（ EC1． 1． 1． 40）催化生成下列反应： Δ G°′ =-0.36千卡。丙酮酸羧化反应（是 H． G． Wood和 C． H． Werkman， 1938最初发现的反应）中有的是苹果酸酶的作用，有的是丙酮酸羧化酶的作用，在生物体内已知以后者的作用为主。两者都是供给三羧酸循环中间体的补充反应
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
ELISA on Attachment-Dependent or Suspension Grown Cells
Enzyme-linked immunosorbant assay (ELISA) has been a powerful technique in immunology since it was first developed in 1971 by Engvall and Perlman (1). The technique is based on the fact that proteins can be absorbed nearly irreversibly to plastic
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