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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
SPATS2-like protein,SPATS2L
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human SPATS2- like protein, SPATS2L ELISA KIT
Product Name:Human SPATS2- like protein, SPATS2L ELISA KIT
Packing:96T
Catalog No.:ELI-18278h
Gene Name:SPATS2L
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:SPATS2-like protein(SPATS2L
Alternative Name:SPATS2L,SPATS2-like protein(SPATS2L
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human SPATS2- like protein, SPATS2L ELISA KIT allows for the in vitro quantitative determination of SPATS2-like protein(SPATS2L concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human SPATS2- like protein, SPATS2L ELISA KIT has been pre-coated with an SPATS2-like protein(SPATS2L antibody specific to SPATS2-like protein(SPATS2L .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SPATS2-like protein(SPATS2L and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SPATS2-like protein(SPATS2L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of SPATS2-like protein(SPATS2L in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Substrate Tablete(antibody developed in goat) St. Louis,Mo 63178, USAAffinity isolated antibody adsorbed Lot # 043H6178With bovine horse and human serum protein Cat # 0h176Lot # 055H4835Lot # A-2179Tween 80Laboratories ,Detroit,Michigan ,USA1 drop of Tween
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Native Chromatin Preparation and Illumina/Solexa Library Construction
carried over through protein-protein interactions. The protocol includes purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP
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