- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-16278h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
TRAF2 and NCK-interacting protein kinase,TNIK
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human TRAF2 and NCK- interacting protein kinase, TNIK ELISA KIT
Product Name:Human TRAF2 and NCK- interacting protein kinase, TNIK ELISA KIT
Packing:96T
Catalog No.:ELI-16278h
Gene Name:TNIK
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:TRAF2 and NCK-interacting protein kinase(TNIK
Alternative Name:TNIK,TRAF2 and NCK-interacting protein kinase(TNIK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human TRAF2 and NCK- interacting protein kinase, TNIK ELISA KIT allows for the in vitro quantitative determination of TRAF2 and NCK-interacting protein kinase(TNIK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human TRAF2 and NCK- interacting protein kinase, TNIK ELISA KIT has been pre-coated with an TRAF2 and NCK-interacting protein kinase(TNIK antibody specific to TRAF2 and NCK-interacting protein kinase(TNIK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TRAF2 and NCK-interacting protein kinase(TNIK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TRAF2 and NCK-interacting protein kinase(TNIK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of TRAF2 and NCK-interacting protein kinase(TNIK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验TRAF‐Mediated TNFR‐Family Signaling
of the protein kinase RIP to the TNF receptor‐1 signaling complex. Immunity 4:387‐396. Hsu, H., Shu, H.B., Pan, M.G., and Goeddel, D.V. 1996b. TRADD‐TRAF2 and TRADD‐FADD interactions
AF006086 181-203 aagaatgaagctgataggacc Human HeLa View, Order View ATR interacting protein (4) AF451323 254-274 AAGGTCCACAGATTATTAGAT Human HeLa View, Order View ATR interacting protein (4)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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