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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Proteasome subunit alpha type- 1, PSMA1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Proteasome subunit alpha type- 1, PSMA1 ELISA KIT
Product Name:Human Proteasome subunit alpha type- 1, PSMA1 ELISA KIT
Packing:96T
Catalog No.:ELI-12789h
Gene Name:PSMA1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Proteasome subunit alpha type-1(PSMA1
Alternative Name:PSMA1,Proteasome subunit alpha type-1(PSMA1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Proteasome subunit alpha type- 1, PSMA1 ELISA KIT allows for the in vitro quantitative determination of Proteasome subunit alpha type-1(PSMA1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Proteasome subunit alpha type- 1, PSMA1 ELISA KIT has been pre-coated with an Proteasome subunit alpha type-1(PSMA1 antibody specific to Proteasome subunit alpha type-1(PSMA1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Proteasome subunit alpha type-1(PSMA1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proteasome subunit alpha type-1(PSMA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Proteasome subunit alpha type-1(PSMA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens cathepsin D (lysosomal aspartyl protease) (CTSD), mRNA 1512 NM_002792 Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 7 (PSMA7), transcript variant 1, mRNA 728 NM_002799 Homo sapiens proteasome (prosome, macropain
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
, native[259]Prolyl 4-hydroxylaseI, α-MF, active437Prostate-specific antigenS, 1.5 mg/L, α-MF, active534, 566Protein kinase C interacting protein 1 (PKCI-1)I, 0.25 mg/L[260]Type 2A Protein Phosphatase, subunit PR65/ASubunit PP2AcI, 5 mg/lI, 100 ug/l(toxic
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