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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Cleavage stimulation factor subunit 1, CSTF1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Cleavage stimulation factor subunit 1, CSTF1 ELISA KIT
Product Name:Human Cleavage stimulation factor subunit 1, CSTF1 ELISA KIT
Packing:96T
Catalog No.:ELI-10131h
Gene Name:CSTF1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cleavage stimulation factor subunit 1(CSTF1
Alternative Name:CSTF1,Cleavage stimulation factor subunit 1(CSTF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cleavage stimulation factor subunit 1, CSTF1 ELISA KIT allows for the in vitro quantitative determination of Cleavage stimulation factor subunit 1(CSTF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cleavage stimulation factor subunit 1, CSTF1 ELISA KIT has been pre-coated with an Cleavage stimulation factor subunit 1(CSTF1 antibody specific to Cleavage stimulation factor subunit 1(CSTF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cleavage stimulation factor subunit 1(CSTF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cleavage stimulation factor subunit 1(CSTF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cleavage stimulation factor subunit 1(CSTF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
【申请上传】Current Protocols in Immunology
and Human Interleukin 9 Unit 6.14 Measurement of Mouse and Human Interleukin 10 Unit 6.15 Measurement of Human Interleukin 11 Unit 6.16 Measurement of Human and Mouse Interleukin-12 Unit 6.17 Measurement of Human and Murine Stem Cell Factor (c-kit Ligand
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