Target Protein Name:Poly(A) RNA polymerase GLD2(PAPD4
Alternative Name:PAPD4,Poly(A) RNA polymerase GLD2(PAPD4 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Poly(A) RNA polymerase GLD2, PAPD4 ELISA KIT allows for the in vitro quantitative determination of Poly(A) RNA polymerase GLD2(PAPD4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Poly(A) RNA polymerase GLD2, PAPD4 ELISA KIT has been pre-coated with an Poly(A) RNA polymerase GLD2(PAPD4 antibody specific to Poly(A) RNA polymerase GLD2(PAPD4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Poly(A) RNA polymerase GLD2(PAPD4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Poly(A) RNA polymerase GLD2(PAPD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Poly(A) RNA polymerase GLD2(PAPD4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!