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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
L- lactate dehydrogenase C chain, LDHC
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human L- lactate dehydrogenase C chain, LDHC ELISA KIT
Product Name:Human L- lactate dehydrogenase C chain, LDHC ELISA KIT
Packing:96T
Catalog No.:ELI-07048h
Gene Name:LDHC
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:L-lactate dehydrogenase C chain(LDHC
Alternative Name:LDHC,L-lactate dehydrogenase C chain(LDHC
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human L- lactate dehydrogenase C chain, LDHC ELISA KIT allows for the in vitro quantitative determination of L-lactate dehydrogenase C chain(LDHC concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human L- lactate dehydrogenase C chain, LDHC ELISA KIT has been pre-coated with an L-lactate dehydrogenase C chain(LDHC antibody specific to L-lactate dehydrogenase C chain(LDHC .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for L-lactate dehydrogenase C chain(LDHC and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain L-lactate dehydrogenase C chain(LDHC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of L-lactate dehydrogenase C chain(LDHC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验(ubiquinol cytochrome c oxido reductase or bc1 complex), IV (cytochrome c oxidase), and V (ATP synthase). These respiratory complexes are conserved from the yeast Saccharomyces cerevisiae to human with the exception of complex I, which is replaced
dehydrogenase (yeast)] 甘油醛磷酸脱氢酶[dlyceraldehyde phosphate dehydrogenase] 原肌球蛋白[tropomyosin] 乳酸脱氢酶[lactate dehydrgenase] 胃蛋白酶[pepsin] 转磷酸核糖基酶[phosphoribosyl transferase] 天冬氨酸氨甲酰转移酶,C链[aspertate transcarbamylase, C chain]
Deglycosylation of Glycoproteins Using Endoglycosidases
enzyme can be added. Note: For human transferrin, PNGase F and Endo F2 release all oligosaccharides in 1 hr. at 37 °C, whereas Endo F3 requires 16 hr. for 50% release of the one oligosaccharide chain. When large scale release of intact
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