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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
C- C chemokine receptor type 5, CCR5
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human C- C chemokine receptor type 5, Ccr5 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
C-C chemokine receptor type 5,C-C CKR-5,CC-CKR-5,CCR-5,MIP-1 alpha receptor,CCR5,chemokine receptor CCR5; CC-CKR-5; CCCKR5; CCR-5; CD195; CKR-5; CKR5; CMKBR5; IDDM22; C-C chemokine receptor type 5; C-C motif chemokine receptor 5 A159A; HIV-1 fusion coreceptor; chemr13; chemokine (C-C motif) receptor 5 (gene/pseudogene)
Search name
Human C-C chemokine receptor type 5 ELISA KIT ,Human C-C CKR-5 ELISA KIT ,Human CC-CKR-5 ELISA KIT ,Human CCR-5 ELISA KIT ,Human MIP-1 alpha receptor ELISA KIT ,Human CCR5 ELISA KIT ,Human chemokine receptor CCR5 ELISA KIT ,Human CC-CKR-5 ELISA KIT ,Human CCCKR5 ELISA KIT ,Human CCR-5 ELISA KIT ,Human CD195 ELISA KIT ,Human CKR-5 ELISA KIT ,Human CKR5 ELISA KIT ,Human CMKBR5 ELISA KIT ,Human IDDM22 ELISA KIT ,Human C-C chemokine receptor type 5 ELISA KIT ,Human C-C motif chemokine receptor 5 A159A ELISA KIT ,Human HIV-1 fusion coreceptor ELISA KIT ,Human chemr13 ELISA KIT ,Human chemokine (C-C motif) receptor 5 (gene/pseudogene) ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human c-c chemokine receptor type 5,C-C CKR-5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-C CKR-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C-C CKR-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C-C CKR-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C-C CKR-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Characterization of Chemokine Receptors
Power, C.A., Meyer, A., Nemeth, K., Bacon, K.B., Hoogewerf, A.K., Proudfoot, A.E.I., and Wells, T.N.C. 1995. Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line. J. Biol. Chem. 270:19495
Determination of HIV-1 Chemokine Coreceptor Tropism Using Transduced Human Osteosarcoma (HOS) Cells
4. Subsequent reports by several laboratories rapidly identified a β-chemokine receptor, CCR5, that mediated entry of macrophage tropic HIV-1 isolates into CD4+ cells (4 –8 ). Since these initial reports, our understanding of HIV-1 cell entry has continued
Anti-c-erb-B-2 Ribozyme for Breast Cancer
Our laboratory has investigated the cleavage activity of an-anti-c-erb -B-2 ribozyme. The c-erb -B-2 proto-oncogene (also called HER-2/neu ) encodes a 185 kDa transmembrane tyrosine kinase-type receptor that is homologous with the epidermal
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