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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海博湖
- 检测范围:
0.312-20ng/ml
- 检测方法:
Sandwich ELISA, Double Antibody
- 应用:
0.188ng/ml
- 样本:
CCR5
- 规格:
96T
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文献和实验volume of 35 ml at room temperature. 2) Add the diluted buffy coat on top of 15 ml of Lymphoprep. 3) Centrifuge at 160 x g for 20 minutes at 20°C. Allow to decellerate without brakes. 4) Remove 20 ml of supernatant
Characterization of Chemokine Receptors
Power, C.A., Meyer, A., Nemeth, K., Bacon, K.B., Hoogewerf, A.K., Proudfoot, A.E.I., and Wells, T.N.C. 1995. Molecular cloning and functional expression of a novel CC chemokine receptor cDNA from a human basophilic cell line. J. Biol. Chem. 270:19495
Determination of HIV-1 Chemokine Coreceptor Tropism Using Transduced Human Osteosarcoma (HOS) Cells
4. Subsequent reports by several laboratories rapidly identified a β-chemokine receptor, CCR5, that mediated entry of macrophage tropic HIV-1 isolates into CD4+ cells (4 –8 ). Since these initial reports, our understanding of HIV-1 cell entry has continued
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