- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-05318h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Sodium- dependent dopamine transporter, SLC6A3
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Sodium- dependent dopamine transporter, SLC6A3 ELISA KIT
Product Name:Human Sodium- dependent dopamine transporter, SLC6A3 ELISA KIT
Packing:96T
Catalog No.:ELI-05318h
Gene Name:SLC6A3
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Sodium-dependent dopamine transporter(SLC6A3
Alternative Name:SLC6A3,Sodium-dependent dopamine transporter(SLC6A3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Sodium- dependent dopamine transporter, SLC6A3 ELISA KIT allows for the in vitro quantitative determination of Sodium-dependent dopamine transporter(SLC6A3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Sodium- dependent dopamine transporter, SLC6A3 ELISA KIT has been pre-coated with an Sodium-dependent dopamine transporter(SLC6A3 antibody specific to Sodium-dependent dopamine transporter(SLC6A3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Sodium-dependent dopamine transporter(SLC6A3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sodium-dependent dopamine transporter(SLC6A3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Sodium-dependent dopamine transporter(SLC6A3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Regulation of Dopamine Transporter Expression by Neuronal Activity
of the transporter protein and mRNA are analyzed by western blots and quantitative RT-PCR, respectively. Finally, methods to study neuronal activity-dependent changes in Ca2+ ⁄calmodulin-dependent protein (CaM) kinase activity in dopamine neurons are described.
Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
Transporter Current Measurements
Na+ /K+ pump, Na+ /Ca2+ exchanger, glutamate transporter and other transporters for dopamine, norepinephrine, GABA, and others, are all electrogenic. These transporters or exchanger transport Na+ ions, with or without transmitters
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