Human Carbohydrate sulfotransf

Human Carbohydrate sulfotransferase 9, CHST9 ELISA KIT

Product Name:Human Carbohydrate sulfotransferase 9, CHST9 ELISA KIT
Packing:96T

Catalog No.:ELI-10649h

Gene Name:CHST9

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:Carbohydrate sulfotransferase 9(CHST9

Alternative Name:CHST9,Carbohydrate sulfotransferase 9(CHST9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human Carbohydrate sulfotransferase 9, CHST9 ELISA KIT allows for the in vitro quantitative determination of Carbohydrate sulfotransferase 9(CHST9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human Carbohydrate sulfotransferase 9, CHST9 ELISA KIT has been pre-coated with an Carbohydrate sulfotransferase 9(CHST9 antibody specific to Carbohydrate sulfotransferase 9(CHST9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Carbohydrate sulfotransferase 9(CHST9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carbohydrate sulfotransferase 9(CHST9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Carbohydrate sulfotransferase 9(CHST9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!