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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Glucagon, GCG
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Glucagon, GCG ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Glucagon,glucagon-like peptide 2; glucagon-like peptide 1; GLP1; GLP2; GRPP; glicentin-related polypeptide,GcG, preproglucagon
Search name
Human Glucagon ELISA Kit, Human glucagon-like peptide 2 ELISA Kit, Human glucagon-like peptide 1 ELISA Kit, Human GLP1
ELISA Kit, Human GLP2 ELISA Kit, Human GRPP ELISA Kit, Human GC ELISA Kit, Human glicentin-related polypeptide ELISA
Kit, Human preproglucagon ELISA KIT,Human GCG ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human glucagon,Glucagon concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glucagon and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Glucagon, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glucagon in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
during the searching procedure. Subsequent alignment might change these scores because of gaps and homologies. A. Result of searching human calmodulin DNA in the EMBL database. The related protein, troponin C, is found in the steep descent of the statistical noise. B
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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