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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Cathelicidin antimicrobial peptide, CAMP
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Cathelicidin Antimicrobial Peptide, CAMP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Human CAMP ELISA Kit,Human Cathelicidin Antimicrobial Peptide ELISA Kit, Human 18 kDa cationic antimicrobial protein ELISA Kit, Human CAP-18 ELISA Kit, Human hCAP-18 ELISA Kit, Human LL-37 ELISA Kit, Human CRAMP ELISA Kit, Human FALL-39 ELISA Kit, Human FALL39 ELISA Kit, Human LL37 ELISA Kit
Intended use
Human CAMP ELISA Kit allows for the in vitro quantitative determination of human cathelicidin antimicrobial peptide concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Human CAMP ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Cathelicidin antimicrobial peptide, During the reaction, Cathelicidin antimicrobial peptide in the
sample or standard competes with a fixed amount of biotin-labeled Cathelicidin antimicrobial peptide for sites on a pre-coated Monoclonal
antibody specific to Cathelicidin antimicrobial peptide. Excess conjugate and unbound sample or standard are washed from the plate.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution
is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cathelicidin antimicrobial peptide in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 125-8000pg/ml
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文献和实验Purification of Antimicrobial Peptides from Human Skin
the identification of human-inducible peptide antibiotics, which are absent in healthy skin. With this strategy, the beta-defensins hBD-2 and hBD-3, RNase-7 as well as psoriasin/S100A7 have been discovered as human antimicrobial peptides and proteins. A detailed
Characterization of the Leishmanicidal Activity of Antimicrobial Peptides
This chapter describes the basic methodology to assay the activity of antimicrobial peptides (AMPs) on Leishmania , a human protozoan parasite. The protocols included can be methodologically divided into two major blocks
dangyonghui2 大侠们,我现在用的是promega公司的cAMP测定试剂盒,按照说明书上面要求做的标准曲线,可是我做了几次出来和说明书上面都不一样,是怎么回事?求解!!! 现在有测cAMP的同志们一起讨论下,我的Q:1186130200 bianbenjamin 我们用的是PE的盒子 LANCETM cAMP 384Kit(PE公司) 1.检测原理:
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