- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-03344h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Pulmonary surfactant- associated protein D, SFTPD ELISA KI
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Pulmonary surfactant-associated protein D, SFTPD ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Pulmonary surfactant-associated protein D,PSP-D,SP-D,Collectin-7,Lung surfactant protein D,surfactant protein DCOLEC7, PSPD,
SFTP4, SP-D, collectin 7, collectin-7, lung surfactant protein D, pulmonary surfactant apoprotein, pulmonary surfactant-associated protein
D, surfactant pulmonary-associated protein D, surfactant-associated protein pulmonary 4, COLEC7,CP4
Search name
Human Pulmonary surfactant-associated protein D ELISA KIT ,Human PSP-D ELISA KIT ,Human SP-D ELISA KIT , Human PSPD
ELISA KIT, Human Collectin-7 ELISA KIT ,Human Lung surfactant protein D ELISA KIT ,Human surfactant protein DCOLEC7
ELISA KIT , Human SFTP4 ELISA KIT ,Human SP-D ELISA KIT ,Human collectin 7 ELISA KIT ,Human collectin-7 ELISA
KIT ,Human lung surfactant protein D ELISA KIT ,Human pulmonary surfactant apoprotein ELISA KIT ,Human pulmonary
surfactant-associated protein D ELISA KIT ,Human surfactant pulmonary-associated protein D ELISA KIT ,Human
surfactant-associated protein pulmonary 4 ELISA KIT,Human COLEC7 ELISA KIT,Human CP4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human pulmonary surfactant-associated protein d,PSP-D
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSP-D. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PSP-D and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain PSP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PSP-D in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Purification of Surfactant Protein D (SP-D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Surfactant protein SP-D is a multimeric collagenous lectin, called collectin . SP-D is a multifunctional, pattern recognition innate immune molecule, which binds in a calcium dependent manner to an array of carbohydrates and lipids
-67 antigen. ref: 1. Gerdes J, Schwab U, Lemke H, Stein H. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31:13–20. 2. The Ki-67 Protein
Isolation and Culture of Human Alveolar Type II Pneumocytes
, through which another crucial function, provision of alveolar surfactant, occurs. Surfactant synthesis and secretion is a unique feature of type II cells; surfactant consists of a high proportion of phospholipids (approx. 90%) and a small proportion of protein (approx. 10
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