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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Major histocompatibility complex class I- related gene pro
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Major histocompatibility complex class I- related gene protein, MR1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MR1,HLALS; MHC class I-like antigen MR-1; MHC class I-related gene protein; MHC class-I related-gene protein; class I histocompatibility antigen-like protein; major histocompatibility complex class I-related gene protein; major histocompatibility complex class I-like sequence; major histocompatibility complex class I-related, Class I histocompatibility antigen-like protein
Search name
Human MR1 ELISA KIT ,Human HLALS ELISA KIT, Human MHC class I-like antigen MR-1 ELISA KIT, Human MHC class I-related gene protein ELISA KIT, Human MHC class-I related-gene protein ELISA KIT, Human class I histocompatibility antigen-like protein ELISA KIT, Human major histocompatibility complex class I-related gene protein ELISA KIT, Human major histocompatibility complex class I-like sequence ELISA KIT, Human major histocompatibility complex class I-related ELISA KIT ,Human Class I histocompatibility antigen-like protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human major histocompatibility complex class i-related gene protein,MHC class I-related gene protein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MHC class I-related gene protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MHC class I-related gene protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MHC class I-related gene protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MHC class I-related gene protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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