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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Osteopontin, SPP1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Osteopontin, SPP1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Osteopontin,;
Uropontin,Urinary stone protein,Secreted phosphoprotein 1(SPP-1),Bone sialoprotein 1,Nephropontin, osteopontin,secreted
phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); osteopontin/immunoglobulin alpha 1 heavy chain
constant region fusion protein; BNSP; BSPI; ETA-1; OPN; SPP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; urinary
stone protein; uropontin; secreted phosphoprotein 1
SearCh name
Human Osteopontin ELISA Kit, Human OPN ELISA Kit, Human Uropontin ELISA Kit, Human Urinary stone protein ELISA Kit,
Human SeCreted phosphoprotein 1 ELISA Kit, Human SPP-1 ELISA Kit, Human Bone sialoprotein 1 ELISA Kit, Human
Nephropontin ELISA Kit, Human bone sialoprotein I ELISA Kit, Human early T-lymphoCyte aCtivation 1 ELISA Kit, Human
immunoglobulin alpha 1 heavy Chain Constant region fusion protein ELISA Kit, Human BNSP ELISA Kit, Human BSPI ELISA Kit,
Human ETA-1 ELISA Kit, Human SPP1 ELISA Kit, Human CALPHA1 fusion ELISA Kit, Human early T-lymphoCyte aCtivation 1
ELISA Kit, Human nephropontin; urinary stone protein ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human osteopontin,Osteopontin concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test prinCiple
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteopontin. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Osteopontin and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Osteopontin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in
color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Osteopontin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
Human Osteopontin, SPP1 ELISA KIT PDF manual download
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
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