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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Heat shock protein beta- 6, HSPB6
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Heat shock protein beta- 6, HSPB6 ELISA KIT
Product Name:Human Heat shock protein beta- 6, HSPB6 ELISA KIT
Packing:96T
Catalog No.:ELI-02939h
Gene Name:HSPB6
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Heat shock protein beta-6(HSPB6
Alternative Name:HSPB6,Heat shock protein beta-6(HSPB6
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Heat shock protein beta- 6, HSPB6 ELISA KIT allows for the in vitro quantitative determination of Heat shock protein beta-6(HSPB6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Heat shock protein beta- 6, HSPB6 ELISA KIT has been pre-coated with an Heat shock protein beta-6(HSPB6 antibody specific to Heat shock protein beta-6(HSPB6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Heat shock protein beta-6(HSPB6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heat shock protein beta-6(HSPB6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Heat shock protein beta-6(HSPB6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
The use of flow cytometry in heat-shock protein (HSP) research is increasing rapidly due to the high sensitivity and versatility of the technique. The method allows the simultaneous analysis of multiple proteins within numerous cell types
Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
to evaluate the cellular capability to cope with exogenous stressors, we investigated stress response profiles in cultured human fibroblasts exposed to short-term heat shock or oxidative stress. Quantitative PCR analysis as well as Western blotting
Quantitation of Heat-Shock Proteins in Clinical Samples Using Mass Spectrometry
capabilities of mass spectrometers. The highly expressed proteins represent potential biomarkers that could aid in diagnosis, therapy, or drug development. Because the proteome is so complex, there is no one standard method for preparing protein samples
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