Human IKBKG ELISA Kit

Human IKBKG ELISA Kit

Product Name:Human IKBKG ELISA Kit
Packing:96T

Catalog No.:ELA-E9868h

Gene Name:IKBKG

Detect Range:0.78-50 ng/mL

Sensitivity:0.34ng/mL

Target Protein Name:Human NF-kappa-B essential modulator

Alternative Name:NF-kappa-B essential modulator, NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma, NF-kappa-B essential modifier
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human IKBKG ELISA Kit allows for the in vitro quantitative determination of Human NF-kappa-B essential modulator concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human IKBKG ELISA Kit has been pre-coated with an IKBKG antibody specific to Human NF-kappa-B essential modulator .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human NF-kappa-B essential modulator and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NF-kappa-B essential modulator, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human NF-kappa-B essential modulator in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!