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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Calcium Sensing Receptor
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Calcium Sensing Receptor ELISA Kit
Product Name:Human Calcium Sensing Receptor ELISA Kit
Packing:96T
Catalog No.:ELA-E9666h
Gene Name:CaSR
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Calcium Sensing Receptor
Alternative Name:CaSR,Calcium Sensing Receptor
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Calcium Sensing Receptor ELISA Kit allows for the in vitro quantitative determination of Calcium Sensing Receptor concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Calcium Sensing Receptor ELISA Kit has been pre-coated with an Calcium Sensing Receptor antibody specific to Calcium Sensing Receptor .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Calcium Sensing Receptor and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Calcium Sensing Receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Calcium Sensing Receptor in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Lectin-Induced Calcium Mobilization in Human Platelets: Use of Fluorescent Probes
The cytosolic calcium concentration, (Ca2+ )c , represents in many types of cells a versatile regulatory system involved in several signal transduction pathways of cell activation (1 ), and is tightly regulated in human platelets
Human primary cytotrophoblast cell culture is a very useful model to study the endocrine and immunological functions of syncytiotrophoblasts, as well as the ion exchange between the mother and her fetus, like calcium. In this chapter
Characterization of Calcium Channel Binding
of the receptor for calcium channel blockers from skeletal muscle. Nature 328:313‐318. Wagner, J.A., Snowman, A.M., Biswas, A., Olivera, B.M., and Snyder, S.H. 1988. ω‐Conotoxin GVIA
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