Human Sphingosine 1 Phosphate

Human Sphingosine 1 Phosphate Receptor 3, S1PR3 ELISA kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Sphingosine 1-phosphate receptor 3(S1P receptor 3,S1P3),;

Endothelial differentiation G-protein coupled receptor 3,Sphingosine 1-phosphate receptor Edg-3(S1P receptor Edg-3),;

Search name

Human Sphingosine 1-phosphate receptor 3 ELISA KIT ,Human S1P receptor 3 ELISA KIT ,Human S1P3 ELISA KIT ,Human Endothelial differentiation G-protein coupled receptor 3 ELISA KIT ,Human Sphingosine 1-phosphate receptor Edg-3 ELISA KIT ,Human S1P receptor Edg-3 ELISA KIT ,Human Endothelial differentiation G-protein coupled receptor 3 ELISA KIT ,Human Lysophospholipid receptor B3 ELISA KIT ,Human S1PR3 ELISA KIT ,Human EDG-3 ELISA KIT ,Human EDG3 ELISA KIT ,Human LPB3 ELISA KIT ,Human S1P3 ELISA KIT ,Human G protein-coupled receptor ELISA KIT ,Human endothelial differentiation gene-3 ELISA KIT ,Human S1P receptor 3 ELISA KIT ,Human S1P receptor EDG3 ELISA KIT ,Human S1P receptor Edg-3 ELISA KIT ,Human endothelial differentiation G-protein coupled receptor 3 ELISA KIT ,Human endothelial differentiation ELISA KIT ,Human sphingolipid G-protein-coupled receptor ELISA KIT ,Human 3 ELISA KIT ,Human sphingosine 1-phosphate receptor 3 ELISA KIT ,Human sphingosine 1-phosphate receptor Edg-3 ELISA KIT ,Human sphingosine-1-phosphate receptor 3 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of human sphingosine 1-phosphate receptor 3,S1P receptor 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1P receptor 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S1P receptor 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain S1P receptor 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of S1P receptor 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF Manual download