Target Protein Name:Human Transcriptional activator Myb
Alternative Name:Transcriptional activator Myb, Proto-oncogene c-Myb, MYB Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human MYB ELISA Kit allows for the in vitro quantitative determination of Human Transcriptional activator Myb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human MYB ELISA Kit has been pre-coated with an MYB antibody specific to Human Transcriptional activator Myb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Transcriptional activator Myb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Transcriptional activator Myb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm