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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
soluble myosin heavy chain 1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078mg/ml
- 规格:
96T
Human soluble myosin heavy chain 1 ELISA Kit
Product Name:Human soluble myosin heavy chain 1 ELISA Kit
Packing:96T
Catalog No.:ELA-E0754h
Gene Name:sMHC-1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:soluble myosin heavy chain 1
Alternative Name:sMHC-1,soluble myosin heavy chain 1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human soluble myosin heavy chain 1 ELISA Kit allows for the in vitro quantitative determination of soluble myosin heavy chain 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human soluble myosin heavy chain 1 ELISA Kit has been pre-coated with an soluble myosin heavy chain 1 antibody specific to soluble myosin heavy chain 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for soluble myosin heavy chain 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain soluble myosin heavy chain 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of soluble myosin heavy chain 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Production of Chimeric Heavy-Chain Antibodies
of fragment crystallizable (Fc), which is known to be crucial for a long serum half-life through binding to FcRn and antibody-mediated cell killing through binding to Fcγ receptors and complement. Single-domain antibodies (sdAbs) derived from camelid heavy
increase in μ heavy-chain mRNA half-life in differentiated B-cells largely accounted for a 100-fold increase in its messenger concentration compared to the undifferentiated cells (3 ). The high specific activity of RNases makes them ideal cellular targets
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
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