Human Activated Protein C, APC

Human Activated Protein C, APC ELISA KIT
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Vitamin K-dependent protein C,Blood coagulation factor XIV,Anticoagulant protein C,Autoprothrombin IIA,;

Search name
Human Protein C ELISA Kit, Human PROC ELISA Kit, Human Vitamin K-dependent protein C ELISA Kit,Human APC ELISA
KIT,Human Activated Protein C ELISA KIT,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Vitamin K-dependent protein C concentrations in serum,
Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin K-dependent protein C. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Vitamin K-dependent protein C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Vitamin K-dependent protein C, biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Vitamin K-dependent protein C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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