- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产(香港）
- CELI-66100h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Dihydrotestosterone, DHT
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human Dihydrotestosterone, DHT ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Dihydrotestosterone,17Beta-hydroxy-5Alpha-androstan-3-one,5Alpha-androstan-17Beta-ol-3-one,androstanolone,DHT,5Alpha-dihydrote
stosterone (5Alpha-DHT),
Search name
Human Dihydrotestosterone ELISA Kit, Human 17Beta-hydroxy-5Alpha-androstan-3-one ELISA Kit, Human
5Alpha-androstan-17Beta-ol-3-one ELISA Kit, Human androstanolone ELISA Kit, Human 5Alpha-dihydrotestosterone ELISA Kit,
Human DHT ELISA Kit, Human 5Alpha-DHT ELISA Kit, Human androstanolone ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of dihydrotestosterone,Dihydrotestosterone concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Dihydrotestosterone, During the reaction, Dihydrotestosterone in the sample or standard
competes with a fixed amount of biotin-labeled Dihydrotestosterone for sites on a pre-coated Monoclonal antibody specific to
Dihydrotestosterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dihydrotestosterone in the samples is then determined by
comparing the O.D. of the samples to the standard curve
PDF manual download
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文献和实验Detection of Ligand-Selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF
, and transcription factors resulting in various degrees of AR activity and stability. The natural ligand of the AR, dihydrotestosterone (DHT), induces a transcriptionally active conformation of the AR while the steroidal antiandrogen cyproterone acetate (CPA
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
提供多种高品质补体相关试剂，助力广大科研工作者的深入探索与研究。 以下是菲恩生物 补体 相关指标产品推荐 ELISA 试剂盒 货号 产品名称 灵敏度 EH5309 Human Anti-C3b antibody ELISA Kit 0.938ng/ml EH5308 Human Anti-CFB antibody ELISA Kit 0.469ng/ml EH5255
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