- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- CELI-66100h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Dihydrotestosterone, DHT
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human Dihydrotestosterone, DHT ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Dihydrotestosterone,17Beta-hydroxy-5Alpha-androstan-3-one,5Alpha-androstan-17Beta-ol-3-one,androstanolone,DHT,5Alpha-dihydrote
stosterone (5Alpha-DHT),
Search name
Human Dihydrotestosterone ELISA Kit, Human 17Beta-hydroxy-5Alpha-androstan-3-one ELISA Kit, Human
5Alpha-androstan-17Beta-ol-3-one ELISA Kit, Human androstanolone ELISA Kit, Human 5Alpha-dihydrotestosterone ELISA Kit,
Human DHT ELISA Kit, Human 5Alpha-DHT ELISA Kit, Human androstanolone ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of dihydrotestosterone,Dihydrotestosterone concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Dihydrotestosterone, During the reaction, Dihydrotestosterone in the sample or standard
competes with a fixed amount of biotin-labeled Dihydrotestosterone for sites on a pre-coated Monoclonal antibody specific to
Dihydrotestosterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dihydrotestosterone in the samples is then determined by
comparing the O.D. of the samples to the standard curve
PDF manual download
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文献和实验Detection of Ligand-Selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF
, and transcription factors resulting in various degrees of AR activity and stability. The natural ligand of the AR, dihydrotestosterone (DHT), induces a transcriptionally active conformation of the AR while the steroidal antiandrogen cyproterone acetate (CPA
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
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