The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
1:500 - 1:2000
IHC
1:50 - 1:200
IF
1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CD4 antibody.
Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody.
Immunohistochemistry of paraffin-embedded rat spleen using CD4 antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using CD4 antibody.
Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody.
Immunofluorescence analysis of HeLa cells using CD4 antibody.
Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody.
Immunofluorescence analysis of PC12 cells using CD4 antibody.
Background:
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Storage:
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.